Primary experimental study on the influence of LPS on osteoblast apoptosis in vitro

【Objective】To study the influence of endotoxin on osteoblast apoptosis in vitro. 【Methods】The mouse calvarial osteoblasts were cultured in vitro to three generations and divided into two groups : the control group and intervention group. Cells of control group were cultured in DMEM/F12 medium containing 10%FBS, to cells of intervention group added 10mmol/L LPS after 1 day normal culture. After 1, 3, 5 and 7 d culture, cells were harvested to measure MTT value. After 3, 7, 10 and 14 d culture, cells were harvested to measure alkaline phosphatase(ALP) and osteocalcin(BGP) activity. After 1 d culture, the osteoblast apoptosis was assessed by flow cytometry. 【Results】There was no significant difference in the value of MTT between two groups(P 0.05). The cellular proliferation rate was significantly lower in intervention group after 3, 5 and 7 d culture(P 0.05 or P 0.01). The values of ALP and BGP were significantly increased in control group and decreased in intervention group after 3, 7, 10 and 14 d culture(P 0.05). The values of ALP and BGP were significantly lower in intervention group than those in control group after 3, 7, 10 and 14 d culture(P 0.01). The apoptotic rate was significantly higher in intervention group than that in control group after 1d culture(P 0.01).【Conclusion】LPS had a marked effect on osteoblast apoptosis by weakening the proliferation and differentiation.