The Relationship between MMP-2,MMP-9 mRNA of Bovine Endometrium and Endometritis

The study was conducted to investigate the role of MMP-2 and MMP-9 in cow endometritis.Healthy and suffering from acute purulent endometritis Chinese Holstein cows were selected as control and experimental group respectively with 10 in each group and all of the cows were 6-10 days postpartum.The concentration of PGF2a,Progesterone was detected by ELISA.CollagenⅠand Ⅳ in endometrium were checked by Immunohistochemistry.MMP-2 and MMP-9 expression were examined using real-time PCR in the endometrium.The results showed that the concentration of PGF2a in experimental group was lower than that in control group(P0.01).The experimental group progesterone was significantly higher than the control group.The expression level of CollagenⅠand Ⅳ in endometristis was higher than the control group(P0.01).The MMP-2 and MMP-9 mRNA expression in endometristis were lower than the control group(P0.01).Conclusion indicated that the expression of MMP-2 and MMP-9 mRNA in cow endometristis reduced,CollagenⅠand Ⅳ degradation were blocked and endometrial cell was unable to secrete PGF2a,making the concentration of progesterone increase in blood serum.MMP-2 and MMP-9 mRNA influenced postpartum endometrial function and encouraged the development of postpartum endometritis.