Regulation effect of resveratrol on hepatic stellate cells in vitro

Objective To investigate the influence of resveratrol on the regulation of hepatic stellate cells(HSCs) in vitro.Methods HSCs were isolated from liver of SD rats.The 5th generation HSCs were cultured on the 96-well plastic plate.The reactive oxygen species in HSCs with resveratrol of different concentration were tested by DCFH-DA kits.The proliferation of HSCs was tested by CCK-8 test kits.Smoothmuscleα-actin(α-SMA) expression of HSCs was evaluated with Western blotting.The activity-related genes were measured with PCR.Results HSCs were isolated from liver and cultured successfully.Resveratrol inhibited the generation of the reactive oxygen species.Proliferation and activation of HSCs were inhibited by resveratrol(P 0.05).Resveratrol inhibited the expression of activity-related genes(MyoD,collagen III and collagen I) in HSCs(P 0.05).Conclusion Resveratrol can inhibit the proliferation and activation of HSCs.Resveratrol maybe has the antioxidative function and could inhibit the expression of MyoD gene.