Research of separation of enantiomers of adrenalines by capillary zone eletrophoresis

Capillary zone electrophoresis(CZE) has been used for the enantiomeric separation of the racemic isoprenaline,noradrenaline and adrenaline with bare fused silica capillary and employing β-cyclodextrin(β-CD) and β-cyclodextrin derivatives as the chiral selectors.Both the complexion and enantiomeric resolution were influenced by β-CD kind,CD concentration and pH of background electrolyte(BGE).The effects of the BGE kinds and concentrations on the enantiomeric separation were also investigated.The results showed that SBE-β-CD has stronger recognition than that of others for the chiral separation of the basic drugs under study.The CD concentrations and pH of BGE have strong influence on the efficiency of chiral separation.Both β-CD and β-cyclodextrin derivatives could not give enantiomeric resolution for noradrenaline and adrenaline.