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The Expression of ICAM-1 mRNA in Neonatal Rats With Hypoxic Ischemic Brain Damage

Ying Xin, Hong Gao, Yu-Kun Han

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Abstract

Objective To study the expression of ICAM 1 at gene transcription level in neonatal rats after hypoxic ischemic brain damage(HIBD). Method Intercellular adhesion molecule 1(ICAM 1) mRNA was detected in cortex of neonatal rats at different times after HIBD and control by reverse transcription polymerase chain reaction(RT-PCR) technique. Result In the ischemic cortex, levels of ICAM 1 mRNA increased markedly at 6 h(2.5 fold, t=2.33, P 0.05), peaked at 24 h(3.6 fold, t=3.37,P 0.01) and remained elevated up to 96 h(2.1 fold, t=2.50,P 0.01) after HIBD. A significant increase in ICAM 1 mRNA expression in the ischemic cortex over levels in contralateral(nonischemic) site was observed( t=2.43, P 0.05). Conclusion ICAM 1 mRNA was upregulated in brain after HIBD. These data support the role of ICAM 1 in mediating leukocyte endothelial adhesion after HIBD in neonatal rats.

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Objective To study the expression of ICAM 1 at gene transcription level in neonatal rats after hypoxic ischemic brain damage(HIBD). Method Intercellular adhesion molecule 1(ICAM 1) mRNA was detected in cortex of neonatal rats at different times after HIBD and control by reverse transcription polymerase chain reaction(RT-PCR) technique. Result In the ischemic cortex, levels of ICAM 1 mRNA increased markedly at 6 h(2.5 fold, t=2.33, P 0.05), peaked at 24 h(3.6 fold, t=3.37,P 0.01) and remained elevated up to 96 h(2.1 fold, t=2.50,P 0.01) after HIBD. A significant increase in ICAM 1 mRNA expression in the ischemic cortex over levels in contralateral(nonischemic) site was observed( t=2.43, P 0.05). Conclusion ICAM 1 mRNA was upregulated in brain after HIBD. These data support the role of ICAM 1 in mediating leukocyte endothelial adhesion after HIBD in neonatal rats.

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Available abstract

Objective To study the expression of ICAM 1 at gene transcription level in neonatal rats after hypoxic ischemic brain damage(HIBD). Method Intercellular adhesion molecule 1(ICAM 1) mRNA was detected in cortex of neonatal rats at different times after HIBD and control by reverse transcription polymerase chain reaction(RT-PCR) technique. Result In the ischemic cortex, levels of ICAM 1 mRNA increased markedly at 6 h(2.5 fold, t=2.33, P 0.05), peaked at 24 h(3.6 fold, t=3.37,P 0.01) and remained elevated up to 96 h(2.1 fold, t=2.50,P 0.01) after HIBD. A significant increase in ICAM 1 mRNA expression in the ischemic cortex over levels in contralateral(nonischemic) site was observed( t=2.43, P 0.05). Conclusion ICAM 1 mRNA was upregulated in brain after HIBD. These data support the role of ICAM 1 in mediating leukocyte endothelial adhesion after HIBD in neonatal rats.

Key concepts: ICAM-1, Messenger RNA, Brain damage, Reverse transcription polymerase chain reaction, Ischemia, Cerebral cortex, Intercellular Adhesion Molecule-1, Internal medicine

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