2013•Western Journal of Traditional Chinese MedicineRequires access
Effects of TangNaiKang on VEGF mRNA Expressions of Human Renal Tubular Epithelial Cells HK-2 Induced by TGF-β_1
Zhu Chun-hu
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Abstract
Objective: To explore the mechanism of TangNaiKang in preventing and treating renal interstitial fibrosis through observing the intervention of TangNaiKang on vascular endothelial growth factor(VEGF) mRNA expressions of human renal tubular epithelial cells(HK-2) induced by transforming growth factor(TGF-β1). Method:HK-2 cells were cultured in the media of DMEM/F12(1:1) containing 10% fetal bovine serum; the cells were divided into six groups: blank control group, TGF-β1group(TGF-β110 ng/mL), blank serum control group(TGF-β110ng/mL+10% blank serum), intervention group one(TGF-β110 ng/mL+5% medicated serum of TangNaiKang),intervention group two(TGF-β110 ng/mL+10% medicated serum of TangNaiKang) and intervention group three(TGF-β110 ng/mL+20% medicated serum of TangNaiKang). After intervened for 24 hours,VEGF mRNA expressions were detected with fluorescence quantitative PCR. Result: VEGF mRNA expressions were raised significantly when HK-2 cells were induced by TGF-β1, it had statistical meaning compared with blank control group(P0.05), VEGF mRNA expressions w ere decreased gradually after intervened with medicated serum of TangNaiKang, it had statistical meaning compared with TGF-β1group(P0.05). Conclusion: TangNaiKang could inhibit VEGF mRNA expressions of HK-2 induced by TGF-β1and the fibrosis of renal tubular epithelial cells to a certain extent.