Effect of Total Flavonoids of Oxytropis falcate on Fibrosis Factor of Human Renal Tubular Epithelial Cell HK-2 Induced by TGF-β _1

Objective: To study the effect of total flavonoids of Oxytropis falcate Bunge on the expression of connective tissue growth factor(CTGF),plasminogen activator inhibitor-1(PAI-1) and matrix metallopeptidase 9(MMP-9) mRNA of human renal tubular epithelial cells HK-2 induced by transforming growth factor-β1(TGF-β1),and to explore the mechanism of total flavonoids of O.falcate on the prevention and treatment of renal fibrosis.Method: The HK-2 cells were cultured by DMEM / F12(1∶ 1) with 10% fetal bovine serum and divided into control group,TGF-β1 10 μg.L-1 group,animal blank serum control group(TGF-β1 10 μg.L-1 + 10% animal serum),total flavonoids of O.falcate-containing serum therapy groups(TGF-β1 10 μg.L-1 + 10% total flavonoids of O.falcate).After 24 h,the expression of CTGF,PAI-1,and MMP-9 mRNA were tested by fluorescence quantitatiye PCR assay.Result: After the HK-2 cell was induced by TGF-β1,the expression of CTGF and PAI-1 mRNA was increased and the expression of MMP-9 mRNA was decreased compared with the control(P 0.05).But the expression of CTGF and PAI-1 mRNA was decreased and the expression of MMP-9 mRNA was increased in HK-2 cultured with TGF-β1 plus total flavonoids of O.falcate compared with only TGF-β1 group(P 0.05),but only rat serum had no such effect.Conclusion: Total flavonoids of O.falcate could prevent the development of renal fibrosis to a certain extent via regulating the expression of fibrosis cell factor.