SH2-B enhances proliferation of colon cancer HT-29 cells

Objective To investigate the effect of SH2-B on proliferation and cell cycle of colon caner HT-29 cells.Methods Screening of the SH2-B low expression colon cancer cell line(HT-29) by immunofluorescence assay was done.pcDNA3.1-SH2-B was transfected into HT-29 cells using LipofecTAMINE 2000.After gene transfection,SH2-B expression was detected by Western-blotting,cell proliferation was measured by MTT assay,and cell cycle was analyzed by flow cytometer.Results Immunofluorescence assay results showed that SH2-B lowly expressed in HT-29 cells.after being transfected with pcDNA3.1-SH2-B,SH2-B expression dramatically increased in HT-29 cells,and HT-29 cells proliferation was significantly enhanced(P0.05).Cell cycle analysis showed that S stage cells were significantly increased in the SH2-B transfected group(39.4±2.31) % compared with the blank control group(18.6±4.25)% and vector control group(11.1±2.39)%(P0.05).Conclusions SH2-B may enhance colon cancer HT-29 cell proliferation and promote cell cycle.