2007•Zhongguo kangfu yixue zazhiRequires access
Effect of nerve growth factor and topiramate on the expression of caspase-3 in the hippocampus in rats with kainic acid induced seizures
Guo Xue-peng
Open publisher page 0 citations
Abstract
Objective: To observe the expression of caspase-3 in hippocampus of epileptic rat induced by kainic acid(KA),and explore the neuroprotective effect of Topiramate(TPM) and nerve growth factor(NGF) to epileptic rat induced by kainic acid.Method: Sixty healthy age from 25 to 35 days rats were randomly divided into 4 groups(n=15): normal sodium(NS) treated as control group(A),KA treated as model group(B),TPM treated as group C,combination of NGF and TPM handled as group D.Five rats in each group were sacrificed by perfusion with Polyoxymethylene respectively on the 1st day,3rd day and 7th day after injection of KA and frozen section were made.The expression of caspase-3 in hippocampus of rats were indicated by immunohistochemical method and quantified by HPIAS-2000.Result: In the same time,the expression of caspase-3 in group D decreased remarkably compared with group B and C(P0.05).Conclusion:TPM can inhibit the expression of caspase-3 caused by epileptic seizure in rat.Combination of TPM and NGF have synergistic effect.