Preparation of immune sera with Schistosoma japonicum 26kDa glutathione s-transferase.

Objective To prepare immune sera with Schistosoma japonicum 26kDa glutathione-s-transferase in rabbits. Methods Schistosoma japonicum 26kDa glutathione s-transferase was purified from induced E.coli JM109 containing recombinant plasmid pGEX-4T-2 lysate;rabbits were immunized with purified Schistosoma japonicum 26kDa glutathione s-transferase on week 0,2 and 6,antibody titer induced by Schistosoma japonicum 26kDa glutathione s-transferase was detected by indirect ELISA with Schistosoma japonicum 26kDa glutathione s-transferase,when the antibody titer of Schistosoma japonicum 26kDa glutathione s-transferase was higher than or equal to 1:6400,the rabbits were killed and their serum samples were collected. Results Schistosoma japonicum 26kDa glutathione s-transferase was purified from E.coli JM109 lysate;immune sera from the rabbits immunized with the protein were obtained and the titer of the antibody induced was 1:12800. Conclusion Immune sera of Schistosoma japonicum 26kDa glutathione s-transferase was obtained by immunizing this protein in rabbits for three times.