Construction of antisense c-myc expression vector and study on the biological effect of transient expression on HepG2.2.15cell in vivo

Objective:To obtain recombinant expression vector pcDNA3.1with c-myc cDNA targeted to hepatocellular carcinoma and construct EGFR mediated hepatocarcinoma targeted gene transfer system-four element complex to study the biological effect of transient expression of antisense c-myc RNA on HepG2.2.15cell.Method:The plasmid pcMYC was excised using XbaI and HindIII and ligated into the same site of pcDNA3.1(-)excised by XbaI and HindIII in antisense directions.GE7and HA20were conjugated with polylysine to produce a conjugates that could in-teract with DNA to form the four element complex.Results:The recombinant pcDNA3.1-as-myc was successfully obtained by the molecular biological technique.After the transient transfection,ex-pression of c-myc protein was inhibited.Con clu sion:The recombinant expression vectors may lead to targeted-expression of c-myc on hepatoma and show tissue specificity and exclusiveness for hep-atoma.