2007•Zhongguo shouyi kexueRequires access

Cloning and sequence analysis of the ITS and 5.8S rDNA of Ancylostoma caninum in China

Zhu Xing-quan

Abstract

The internal transcribed spacer(ITS) and 5.8S rDNA of Ancylostoma caninum isolated in Guangdong Province of China were amplified by PCR using a pair of conserved primers and the amplicons were cloned into pGEM-T Easy vector,respectively.The inserts were successfully sequenced,and the ITS of the two A.caninum samples was 738 bp in length.Sequence analysis revealed that the ITS-1,5.8S and ITS-2 rDNA of both A.caninum samples were 364 bp,153 bp and 221 bp in length,respectively,and polymorphism was found in the ITS-1 and ITS-2 rDNA sequences between the two A.caninum samples.The results provided a foundation for further studies of molecular identification and molecular genetics of A.caninum.

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Key concepts: Ancylostoma caninum, Biology, Amplicon, Internal transcribed spacer, Sequence analysis, Genetics, Cloning (programming), Ribosomal DNA