Screening of HIV-1 gp41 CHR mimotope from phage displays peptide library

Objective To identify and characterize the HIV 1 gp41 CHR mimotope, and to explore whether the mimotopes can be used as inhibitor for blocking the binding of gp41 NHR and CHR.Methods The mimotopes of HIV 1 gp41 CHR were screened from 12 mer phage display peptide library by using McAb 1G1 against HIV 1 gp41 C34mer synthetic peptide in CHR, and identified by sandwich ELISA.Results 6 of 17 phage clones were identified as positive clones which can bind to IG1 mAb and HIV 1 gp41 synthetic peptide N36 specifi cally .The amino acid sequence in all of these 6 clones is the same,HYEFWAWNWEAN, which is hydrophobic as N terminal of C34.Conclusion This phage display peptide can mimic the epitope of HIV 1 gp41 CHR ,and bind with HIV 1 gp41 NHR.