Determination of Glucosamine in Mycelium of Hericium erinaceus

Objective To ascertain the relationship between the content of glucosamine in mycelium of Hericium erinaceus and the culture medium and the time.Methods Digest the mycelium of Hericium erinaceus,neutralize it and show colour,then determine the content of glucosamine by spectrophotometric method.Results The content of glucosamine in mycelium of Hericium erinaceus was different in different cultural.medium.It was the highest in mycelium of Hericium erinaceus in culture potato,and it is close in culture wheat and culture corn,it is tallish in culture corn.The content of glucosamine in mycelium of Hericium erinaceus of different culture time is different,but the difference is not distinct between the 25th day and the 35th day.Conclusion To determine the content of mycolium of Hericium erinaceus,we can use the puremycelium cultured at the similar condition with solid culture medium as reference,and the culture time between 25 days and 35 days is available.