Tissues expression of human era gene mRNA

AIM To examine the expression levels of human er a mRNA in different tissues or tumor cell lines. METHODS Human era encoding region gene labeled with (dCTP was used as a probe and its mRNA levels were examined in multiple tissue northern (MTN) blot membrane and multiple tissue expression (MTE) array membrane by Northern blot and Dot blot h ybridization respectively. RESULTS Northern blot showed the pro be identified an mRNA product of approximately 2.2 kb, and Dot blot showed human era expressed in all tissues and cell lines, the highest expression found i n the nervous system, some fetal tissues and tumor cell lines. CONCLUSIO N Human era mRNA expresses in all tissues tested at different level s, so it may be a constitutively expressing gene.