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2004•Chinese Journal of Veterinary DrugRequires access

Determination of oxytetracycline residues in milk by RP-HPLC

WU Xu-gang

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Abstract

The method for determining oxytetracycline residues in milk by RP-HPLC was established. Oxytetracycline in milk was extracted by methanol with oxalic acid. After being centrifuged, the extract was assayed by HPLC with C_(18) column(5 μm,250 mm×4.6 mm) and methanol-acetonitrile - 0.01mol/L oxalic acid (20∶12∶68,(V/V)) as the mobile phase at flow rate of 1 mL/min. The detection wavelenght was at 360 nm. The standard curve was linear for oxytetracycline in the range of 50～1 500 μg/L(r=0.999 9,n=6). The average recovery(n=5) was 88%. The method is simple, accurate and rapid.

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What this paper is about

The method for determining oxytetracycline residues in milk by RP-HPLC was established. Oxytetracycline in milk was extracted by methanol with oxalic acid. After being centrifuged, the extract was assayed by HPLC with C_(18) column(5 μm,250 mm×4.6 mm) and methanol-acetonitrile - 0.01mol/L oxalic acid (20∶12∶68,(V/V)) as the mobile phase at flow rate of 1 mL/min. The detection wavelenght was at 360 nm. The standard curve was linear for oxytetracycline in the range of 50～1 500 μg/L(r=0.999 9,n=6). The average recovery(n=5) was 88%. The method is simple, accurate and rapid.

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Available abstract

The method for determining oxytetracycline residues in milk by RP-HPLC was established. Oxytetracycline in milk was extracted by methanol with oxalic acid. After being centrifuged, the extract was assayed by HPLC with C_(18) column(5 μm,250 mm×4.6 mm) and methanol-acetonitrile - 0.01mol/L oxalic acid (20∶12∶68,(V/V)) as the mobile phase at flow rate of 1 mL/min. The detection wavelenght was at 360 nm. The standard curve was linear for oxytetracycline in the range of 50～1 500 μg/L(r=0.999 9,n=6). The average recovery(n=5) was 88%. The method is simple, accurate and rapid.

Key concepts: Oxytetracycline, High-performance liquid chromatography, Chromatography, Chemistry, Oxalic acid, Methanol, Acetonitrile, Antibiotics

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