Influence of high-fat diet on levels of serum lipid and TNF-α in rats with obesity prone and obesity resistance

Objective: To study the influence of high-fat diet on levels of serum lipid and tumor necrosis factor-α (TNF-α) in rats with obesity prone and obesity resistance. Methods: Male Sprague-dawley rats were divided into control group and high-fat group randomly,maintained respectively a standard and a high-fat diet for 14 weeks. Then the rats of high-fat group were divided into 2 groups according to the body weight. The levels of serum total cholesterol(TC),triglyceride(TG),high density lipoprotein-C(HDL-C) and TNF-α were determined at the 1st,3rd,14th week. Results: After 3 weeks,the TC,TG values of OP group were significantly increased compared with OR and control groups( P 0.05),its HDL-C values were significantly decreased compared with control group ( P 0.05). At the end of the experiment,the TG,HDL-C and TNF-α levels of OP group differed from the OR and control groups ( P 0.01),the TC levels of OP and OR groups were significantly increased compared with the control ( P 0.01). The TNF-α levels in OR rats were significantly higher than control rats ( P 0.05). Conclusion: The high-fat diet may induce hypercholesterolemia but not hypertriglyceridemia. It could influence the occurrence of obesity and its complications by changing serum TNF-α levels in rats.