2003•Zhonghua weishengwuxue he mianyixue zazhiRequires access
Preparation of polycolonal anti-LPS antibody with cross reactivity and screening of mimotopes of LPS from phage displayed peptide library
Shangen Zheng, Beiyi Liu, Ping Zhu, Ning Fu
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Abstract
Antisera can react with different LPS and protect mice from infection by E. coli and P. pyocyanea. After 3 round of screening, 20 of 46 clones were identified as positive which can bind to polyclonal antibody. The IC_(50) of all positive clones was about 125ng/ml of LPS of S. typhimurium. 10 positive clones were sequenced and the amino acid sequences were deduced, in which 5 clones showed sequence as X-QFYP-X-A, 3 clones as LFTFAHYA and 2 clones as YQYYPAA. The average content of hydrophobic amino acid was 80.0%. Polyclonal anti-LPS antibody with cross reactivity and protection was obtained, and mimotope clones of LPS were screened from phage displayed peptide library. These phage clones can bind to anti-LPS antibody like LPS, which suggests that the peptide displayed on phage can mimic epitope of LPS.