Antisense oligonucleotide targeting survivin inducing apoptosis of renal cancer cell line 786-O in vitro

Objective To investigate the effect of antisense oligonucleotide (ASODN) targeting survivin on the apoptosis and proliferation of renal cancer cell line 786-O. Methods ASODN targeting survivin was designed and constructed.Cultured cells were divided into 6 groups:control group,liposome group, sense oligonucleotide (SODN) group,200 nmol/L ASODN group,400 nmol/L ASODN group and 600 nmol/L ASODN group.Cells were transfected for 24 h and then harvested to carry on the next tests.Morphological changes of cells were observed by transmission electron microscope. Survivin protein expression was detected by immunohistochemical method and imaging analysis. Apoptosis index (AI) and cell cycle were examined by flow cytometry. Inhibition ratio (IR) was determined by the colorimetri MTT cell viability and proliferation assay. Results Abnormal morphological changes of cells were observed in ASODN transfected groups.Expression of survivin in ASODN groups was significantly decreased compared with control group,liposome group and SODN group.The AI of ASODN groups was significantly higher than that of other groups.The IR of ASODN groups was significantly higher than that of other groups. Conclusion Expression of survivin may decrease 786-O cells after ASODN transfection.ASODN targeting survivin can induce 786-O cell apoptosis and inhibit 786-O cell proliferation.