2011•Journal of environmental healthRequires access

Pulsed-field Gel Electrophoresis Fingerprint Analysis of Legionella pneumophila in Water from Centralized Air Conditioning System in Shijiazhuang

Zhou Ji-kun

Abstract

Objective To analyze the molecular types of Legionella(L.)pneumophila strains isolated from water in centralized air conditioning system(CACS) in Shijiazhuang and to develop the PulseNet-Shijiazhuang Database of L.pneumophila.Methods Pulsed field gel electrophoresis(PFGE) was used to analyze L.pneumophila collected from water in CACS of Shijiazhuang in 2008.After digesting with AscI,BioNumerics(Version 4) software was used to analyze the PFGE fingerprints.Cluster analysis was performed using the Marker H9812 calibration method.Results Twenty-five strains of L.pneumophila were divided into 13 banding patterns,with SJZLP0006(7 isolates) banding pattern strains accounted for 28% of bacterial strains.Cluster diagram showed all the serotype 1 strains similarity coefficients were 60%-100%;serum type 2-14 strains were 80%-100%.Conclusion L.pneumophila strains isolated from water in CACS in Shijiazhuang are with polymorphism in distribution and high genetic variations and also have the characteristics of cloning.

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Key concepts: Legionella pneumophila, Pulsed-field gel electrophoresis, Serotype, Microbiology, DNA profiling, Legionnaires' disease, Biology, Legionella