Isolation and identification of bacteria in the intestinal tract of adult Bactrocera tau(Walker)(Diptera:Tephritidae)
Luo Mijua
Abstract
Luo Mijua
Abstract
21 strains of bacteria were isolated and identified from the intestinal tract of sexually matured female Bactrocera tau which were reared in the laboratory using traditional isolation and 16 S r DNA( V6- V8 hypervariable region) sequencing technique. All bacteria strains belonged to the Gamma-proteobacteria phylum,which included Enterobacter( 7 strains),Raoultella( 3 strains),Serratia( 2 strains),Providencia( 2 strains),Acinetobacter( 1 strain),Citrobacter( 1 strain),Cedecea( 1 strain) and uncultured bacteria( 4 strains). Similarly,20 bacteria strains were isolated and identified from male B. tau and they also belonged to Gammaproteobacteria phylum,which consisted of bacteria( 7 strains),Klebsiella( 4 strains),Enterobacter( 3 strains),Providencia( 2strains),Serratia( 1 strain),Morganella( 1 strain),Pseudomonas( 1 strain) and gamma Proteobacterium( 1 strain). Bacteria genus which both females and males had in common were Enterobacter,Providencia and Serratia,which all belonged to Enterobacteriaceae. To summarize,Enterobacteriaceae was the dominant bacterium in intestinal tract of B. tau.
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21 strains of bacteria were isolated and identified from the intestinal tract of sexually matured female Bactrocera tau which were reared in the laboratory using traditional isolation and 16 S r DNA( V6- V8 hypervariable region) sequencing technique. All bacteria strains belonged to the Gamma-proteobacteria phylum,which included Enterobacter( 7 strains),Raoultella( 3 strains),Serratia( 2 strains),Providencia( 2 strains),Acinetobacter( 1 strain),Citrobacter( 1 strain),Cedecea( 1 strain) and uncultured bacteria( 4 strains). Similarly,20 bacteria strains were isolated and identified from male B. tau and they also belonged to Gammaproteobacteria phylum,which consisted of bacteria( 7 strains),Klebsiella( 4 strains),Enterobacter( 3 strains),Providencia( 2strains),Serratia( 1 strain),Morganella( 1 strain),Pseudomonas( 1 strain) and gamma Proteobacterium( 1 strain). Bacteria genus which both females and males had in common were Enterobacter,Providencia and Serratia,which all belonged to Enterobacteriaceae. To summarize,Enterobacteriaceae was the dominant bacterium in intestinal tract of B. tau.
Key concepts: Providencia, Citrobacter, Serratia, Microbiology, Enterobacter, Biology, Bacteria, Enterobacteriaceae