Simultaneous determination by HPLC of four kinds of antibiotics in seafood

A method for simultaneous determination of oxytetracycline,tetracycline,chlortetracycline and choramphenicol in seafood was successfully developed using high performance liquid chromatography with ammonium acetate/water and acetonitrile as the mobile phase.The results showed that the four antibiotics were well separated under the optimum conditions,with the limits of detection being 32μg/kg for oxytetracycline,15μg/kg for tetracycline,23μg/kg for chlortetracycline,and 19μg/kg for choramphenicol.The recovery of standard additions was 76%～92% and the relative standard deviation was 0.8%～4.5%.The method is rapid,sensitive,accurate and reliable and can be applied in the analysis of antibiotic residues in seafood.