The influence of Akt1 siRNA on the proliferation and apoptosis of human laryngeal carcinoma cell line

Objective:To investigate the effect of siRNA targeting Akt1 gene on the proliferation and apoptosis of cultured human laryngeal carcinoma cell line.Methods:siRNA targeting Akt1 was designed and transfected into Hep-2 cell.At 48 hours after transfection,RT-PCR and western blot technique were used to test the inhibitory effect of Akt1 siRNA.The activity of cell proliferation was examined by MTT assay and survival rate was calculated after trypan blue staining.Cell apoptosis and necrosis were tested with AnnexinV/PI kit.Results:The Akt1 siRNA designed could interfere the Akt1 expression of Hep-2.The mRNA and protein expression level of Akt1 gene of Akt1 siRNA group were lower than that of blank and negative control group;The proliferative activity was inhibited,survival rate was decreased,cell apoptosis and necrosis rate increased after Akt1siRNA transfection.Conclusion:RNA interference can inhibit cell proliferation and induce apoptosis of laryngeal carcinoma cell line.Akt1 may be a novel target in the gene therapy of laryngeal carcinoma.