Identification of Endophytic Bacterium Strain B_(47) and Its Control Effects on Tomato Bacterial Wilt

Tomato bacterial wilt is an important plant soilborne disease. The endophytic bacterium strain B_(47) isolated from stem of tomato could strongly inhibit the growth of .Ralstonia solanacearum.. This strain was identified as Bacillus subtilis according to its morphological, physiological and biochemical characteristics and 16S rDNA sequence analysis. The optimum pH and temperature for the growth of the strain were 5-6 and 35 respectively. The results of control tests in greenhouse showed that 81.25% and 92.10% of control effects on tomato bacterial wilt could be obtained when strain B_(47) was inoculated to tomato in advance of Ralstonia solanacearum. If strain B_(47) and Ralstonia solanacearum were inoculated to tomato simultaneously, the strain B_(47) could not effectively control the disease.