ScholarLens Back to search
2001•China Public HealthRequires access

Study on Cytotoxic Effects of EHECO_(157)∶H_7 (Zhejiang Strain) and its Significance

Wen Zhang

Open publisher page 0 citations

Abstract

Objective To determine the cytotoxicity of EHEC O\-\{157\}∶H\-7 97-1-68 strain (Zhejiang strain) with Vero,HeLa,CHO,and KMB\-\{17\} cell-lines and compare it with other strains. Methods Bacterial supersonic lysates and culture supernatants were tested for cytotoxic activity with monolayer cells in microtitre tissue culture trays.Results The cytotoxicities of 97-1-68 strain were negative all against the four cell-lines while the positive reference strains had high titers of cytotoxicity against Vero and HeLa cell-lines.Conclusion A viewpoint on Two Sorts of Strains in EHEC O\-\{157\}∶H\-7 infections was put forward.

About this research paper

What this paper is about

Objective To determine the cytotoxicity of EHEC O\-\{157\}∶H\-7 97-1-68 strain (Zhejiang strain) with Vero,HeLa,CHO,and KMB\-\{17\} cell-lines and compare it with other strains. Methods Bacterial supersonic lysates and culture supernatants were tested for cytotoxic activity with monolayer cells in microtitre tissue culture trays.Results The cytotoxicities of 97-1-68 strain were negative all against the four cell-lines while the positive reference strains had high titers of cytotoxicity against Vero and HeLa cell-lines.Conclusion A viewpoint on Two Sorts of Strains in EHEC O\-\{157\}∶H\-7 infections was put forward.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Objective To determine the cytotoxicity of EHEC O\-\{157\}∶H\-7 97-1-68 strain (Zhejiang strain) with Vero,HeLa,CHO,and KMB\-\{17\} cell-lines and compare it with other strains. Methods Bacterial supersonic lysates and culture supernatants were tested for cytotoxic activity with monolayer cells in microtitre tissue culture trays.Results The cytotoxicities of 97-1-68 strain were negative all against the four cell-lines while the positive reference strains had high titers of cytotoxicity against Vero and HeLa cell-lines.Conclusion A viewpoint on Two Sorts of Strains in EHEC O\-\{157\}∶H\-7 infections was put forward.

Key concepts: HeLa, Vero cell, Cytotoxicity, Strain (injury), Cytotoxic T cell, Titer, Cell culture, Microbiology

Back to paper searchBrowse research topicsOriginal source
Study on Cytotoxic Effects of EHECO_(157)∶H_7 (Zhejiang Strain) and its Significance — Research Paper | ScholarLens