Studies on the Level of Microtubule-associated Protein Tua in Neonatal Rats with Hypoxic-ischemic Brain Damage

Objective:This study used the classic hypoxic-ischemic brain damage(HIBD) amlimal models to observe the changes of tau protein levels in periventricular white matter before and after hypoxic-ischemic brain damage(HIBD),and to explore the relationship between tau protein expression changes and brain damage in early neonatal rats with hypoxic-ischemic brain damage(HIBD).Methods:Cleanly 7-day-old Wistar rats were randomly divided into sham operation grup(the control group,n=40) and hypoxic-ischemic brain damage model group(HIBDgroup n=42).In the light of Rice,unilateral ligated left common carotid artery of neonatal rats to make HIBD animal model;sham operation group(control group) just cut the middle of the neck skin,isolated but not ligated the left common carotid artery;HIBD group and control group of rats were put into Hypoxic hypoxia environment 2 hours and then were randomly diVided into 3h,6h,12h,24h,48hgroup(n=8).After hematoxylin and eosin staining and silver staining,using microscope observing the pathological change of neuron in newborn rats before and after HIBD at differen times;immunohistochemical observing dynamic chanaes of microtubule-associated protein Tau level at periventricular white matter and cortical of rats,400 times in the view,using of computer image analysis system measured the average of Tau protein optical denslty and statistcally analyzed.Results:The silver staining showed cerebral tissue with hypoxic-ischemic brain damage neonatal rats neurohbhllary tangles and aopotostic cells can be seen in periventricular alba and cerebral cortex.Comparing with the sham group,immunoreactivity of Tau in different HIBD groups was increased with prolongation of ischemia.3 hours after HI were not signillcantly different compared with the control group,OD value of 6h group,12h group,24h and 48h group after HIBD increased signincantly compared with the control group(p0.05),there was a signincant difference.Conclution:HIBD can cause nerve fiber degeneration in the brain periventricular white matter of neonatal rats,the level of Tau protein increased.