Effects of Breviscapine on intracellular Ca~(2+) concentration in human umbilical vein endothelial cells

Aim To investigate the effects of Breviscapine(BE)on intracellular Ca2+ concentration in human umbilical vein endothelial cells(HUVECs). Methods HUVECs were incubated with the calcium ion-sensitive fluorescent indicator fluo-3/AM, and then a laser confocal microscope was applied to measure changes of fluorescence intensity under different agonists to investigate the effects of Breviscapine on intracellular Ca2+ concentration in HUVECs. Results ①BE induced Ca2+ transients in the buffer with or without Ca2+;②The Ca2+ transients induced by BE could be overlapped by cyclopiazonic acid (CPA);③Pretreatment of BE inhibited Ca2+ influx caused by KCl.④ BE had no evident effect on the calcium influx in- duced by the restoration of extracellular Ca2+ after the depletion of intracellular Ca2+ stores.Conclusion BE depletes CPA-sensitive intracellular Ca2+ stores and inhibits Ca2+ influx through voltage-dependent Ca2+ channels, and BE has no evident effect on the calcium influx induced by the restoration of extracellular Ca2+ after the depletion of intracellular Ca2+ stores.