Effect of insulin-like growth factor 1 receptor α-strain interrupted on lung adenoma cell line SPC-A-1

Purpose:To study the effect of insulin-like growth factor 1 receptor α-strain(IGF1R-α) interrupted by a special antibody (IGFⅠR-αMab)on lung adenoma cell line SPC-A-1.Methods:IGFⅠR-αMab was extracted by hybrid technology. SPC-A-1 cells were separated into 2 groups,the IGFⅠR-αMab and the blank control. The IGFⅠR-αMab cells were interfered by different densities of IGFⅠR-αMab, including 20,40,60,80,100,120,140,160,180 and 200 ng/ml. The MTT curve line, morphology, ultrastructure and cell cycles were observed at 0,24,48,72 hours after the intervention respectively. Results:Compared with the control, apoptosis in IGFⅠR-αMab group was significant(P= 0.009)and proliferation rate was decreased obviously within 160 ng/ml. However, the proliferation rate was no significant when the special antibody density was more than 200 ng/ml.Conclusions:The affinity of IGFⅠR-αMab at IGF1R α-strain is high. The interruption of IGF1R α-strain by IGFⅠR-αMab shows the obvious biological effects in vitro ,with inclusion of promoting apoptosis and suppressing proliferation, which indicate the interruption targeting IGF1R α-strain is prospective for non-small-cell lung carcinoma.