2012Northern HorticultureRequires access

Study on Differences of The Amylase Activity in Different Raphanus sativus L.

Chang Liu

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Abstract

To determine theα,β-amylase activity and total amylase activity in 25radish varieties,field experiments used randomized block design and repeated 3times,α?amylase,β-amylase and total amylase activity were estimated by the 3,5-dinitrosalicylic acid method after fleshy root harvest,for providing a reference and establishment relationship of α?amylase,β-amylase and total amylase activity.The results showed that there were significance differences ofα?amylase,β-amylase and total amylase activity in different radish varieties;the A04,S01,A17,A20,S05and A07were the higher α?amylase activity and the A19,A06,A17,A12and S03were the the higher β-amylase and total amylase activity.The ratio ofα,β-amylase activity in total amylase activity was 8.11%to 25.57% and 75.26%~91.89%,respectively and the relationship of them was Y Total amylase activity =-0.029+1.039X α-amylase activity +0.998X β-amylase activity.

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What this paper is about

To determine theα,β-amylase activity and total amylase activity in 25radish varieties,field experiments used randomized block design and repeated 3times,α?amylase,β-amylase and total amylase activity were estimated by the 3,5-dinitrosalicylic acid method after fleshy root harvest,for providing a reference and establishment relationship of α?amylase,β-amylase and total amylase activity.The results showed that there were significance differences ofα?amylase,β-amylase and total amylase activity in different radish varieties;the A04,S01,A17,A20,S05and A07were the higher α?amylase activity and the A19,A06,A17,A12and S03were the the higher β-amylase and total amylase activity.The ratio ofα,β-amylase activity in total amylase activity was 8.11%to 25.57% and 75.26%~91.89%,respectively and the relationship of them was Y Total amylase activity =-0.029+1.039X α-amylase activity +0.998X β-amylase activity.

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Available abstract

To determine theα,β-amylase activity and total amylase activity in 25radish varieties,field experiments used randomized block design and repeated 3times,α?amylase,β-amylase and total amylase activity were estimated by the 3,5-dinitrosalicylic acid method after fleshy root harvest,for providing a reference and establishment relationship of α?amylase,β-amylase and total amylase activity.The results showed that there were significance differences ofα?amylase,β-amylase and total amylase activity in different radish varieties;the A04,S01,A17,A20,S05and A07were the higher α?amylase activity and the A19,A06,A17,A12and S03were the the higher β-amylase and total amylase activity.The ratio ofα,β-amylase activity in total amylase activity was 8.11%to 25.57% and 75.26%~91.89%,respectively and the relationship of them was Y Total amylase activity =-0.029+1.039X α-amylase activity +0.998X β-amylase activity.

Key concepts: Amylase, Raphanus, Chemistry, Food science, Enzyme, Biology, Botany, Biochemistry

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