Construction of eukaryotic expression vector for KGPcd gene and its expression in vitro

AIM:To construct eukaryotic expression vector of KGPcd gene encoding whole amino acid residues of mature KGPcd from Porphyromonas gingivalis and to investigate the transcription and expression of recombined plasmid pcDNA3.1(+)/ KGPcd .METHODS:The pcDNA3.1(+)/ KGPcd was transfected into mammalian cell COS7 with Lipofectamine 2000.The transcription of pcDNA3.1(+)/ KGPcd was assayed by reverse transcription polymerase chain reaction (RT-PCR). The expression product of pcDNA3.1(+)/ KGPcd was detected by method of indirect immunofluorescent.RESULTS:The eukaryotic expression vector of KGPcd gene was constructed successfully. It proved that the product of pcDNA3.1(+)/ KGPcd was expressed in transcripted COS7 cells.CONCLUSION:The recombinant plasmid pcDNA3.1(+)/ KGPcd could be transcripted and expressed correctly in mammalian cell COS7 in vitro .