Mechanism of H-7 in brain injury after intracerebral hemorrhage

Objective To investigate the mechanism of protein kinase C(PKC) inhibitor H-7 in brain injury after intracerebral hemorrhage.Methods SD rats were divided randomly into 4 groups: normal saline group﹑sham-operated group﹑ICH group﹑H-7 group.Autologous blood were injected into the right caudate nucleus in the ICH groups,H-7 was injected into the intraperitoneal every 12 h in the H-7 group.The permeability of blood brain barrier(BBB) was evaluated by Evans blue(EB) contents and the water content of brain was measured by comparion of wet and dry weight in perihematoma,and histopathological appearance was observed.Results H-7 significantly reduced the permeability of BBB(P0.05),and decreased significantly the water content of brain(P0.05) consequently in rats after intracerebral hemorrhage,as well as inflammatory cell infiltrate.Conclusion PKC inhibitor H-7 can significantly decrease the brain edema﹑the damages of the permeability of BBB and inflammatory cell infiltrate.PKC take an important role in brain damage after ICH.