Studies on meiosis characteristic and fertility restoration of male sterile wheat with Aegilops kotschyi cytoplasm

The frequency of univalent,lagging chromosomes and micronuclei in tetrad in PMC of the 1B/1R and non-1B/1R CMS lines of wheat with Ae.kotschyi cytoplasm,and their maintainer and their hybrid F 2,were investigated.The results were as follows:(1) The affects of Ae.kotschyi cytoplasm for on the chromosomes pairing were different in special 1B/1R CMS lines.(2) It was found that the frequency of MI cells with univalents of the five CMS lines were correlated to the frequency of cells with lagging chromosomes and the frequency of AI cells with micronuclei in tetrad,nor were their maintainer lines.(3) The affect of Ae.kotschyi cytoplasm on non-1B/1R CMS lines is not obvious,the interacts of special 1B/1R nuclei and Ae.kotschyi cytoplasm was the reason of abnormal meiosis.(4) Frequency of change in this three period is opposite to fertility restoration of their F 2,the abnormal meiosis was one of the important reason that the fertility restoration of 1B/1R CMS lines was low and easy to change.