CTLA4Ig fusion protein expression plasmid transfer into Wistar allo-grafted skins and cells by gene-gun

Objective To observe the efficiency of CTLA4 gene transfer by gene gun into allo skin samples and cultured cell.Methods A recombinant vector carrying CTLA4Ig IRES2 EGFP gene was constructed and then transferred into allo skin samples by gene gun or intracutaneous injection or muscle injection.Moreover, cultured cells were also transferred with CTLA4Ig expression vectors by gene gun or by incubation was.After 24,72 hrs, transfection efficacy and expression of the recombinant vector carrying CTLA4Ig IRES2 EGFP gene were determined by immunocytochemistry.Results All the three methods of transfection carry the plasmid into skin and cultured cells, but direct transfer of naked DNA of the plasmid using gene gun is more feasible. For cultured cells, it was esasier to die if the gene gun at too high pressure.Conclusion The gene transfer of CTLA4Ig IRES2 EGFP into skin samples can be more effective than others by gene gun.