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2001•Chinese Journal of Hospital PharmacyRequires access

Determination of cortisol and cortisone in urine by HPLC

Zhamg Yin

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Abstract

OBJECTIVE:To investigate 11β hydroxystroid dehydrogenase activity,an exact precision reproducible assay was developed for measuring the ratio of cortisol and cortisone.METHODS:Gradient HPLC was used.RESULTS:The linear regress equations for cortisol and cortisone were Y = 0.01 X - 0.027 and Y = 0.012 X - 0.025 ,the percentages of their average recovery were ( 105.8 ± 3.1 )% and ( 99.6 ± 3.6 )%( n =15, RSD : 2.40% ～ 4.87% ) respectively.CONCLUSIONS:This method is able to satisfy demands for measuring glucocorticoid of human urine,and it is very sensitive and credible.The concentration of cortisol and cortisone in 34 healthy volunteers's urine were ( 25.2 ± 14.3 )ng·ml -1 and 46.2 ± 26.7 ng·ml -1 and the ratio of cortisol and cortisone was 0.59 ± 0.18 .

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OBJECTIVE:To investigate 11β hydroxystroid dehydrogenase activity,an exact precision reproducible assay was developed for measuring the ratio of cortisol and cortisone.METHODS:Gradient HPLC was used.RESULTS:The linear regress equations for cortisol and cortisone were Y = 0.01 X - 0.027 and Y = 0.012 X - 0.025 ,the percentages of their average recovery were ( 105.8 ± 3.1 )% and ( 99.6 ± 3.6 )%( n =15, RSD : 2.40% ～ 4.87% ) respectively.CONCLUSIONS:This method is able to satisfy demands for measuring glucocorticoid of human urine,and it is very sensitive and credible.The concentration of cortisol and cortisone in 34 healthy volunteers's urine were ( 25.2 ± 14.3 )ng·ml -1 and 46.2 ± 26.7 ng·ml -1 and the ratio of cortisol and cortisone was 0.59 ± 0.18 .

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Available abstract

OBJECTIVE:To investigate 11β hydroxystroid dehydrogenase activity,an exact precision reproducible assay was developed for measuring the ratio of cortisol and cortisone.METHODS:Gradient HPLC was used.RESULTS:The linear regress equations for cortisol and cortisone were Y = 0.01 X - 0.027 and Y = 0.012 X - 0.025 ,the percentages of their average recovery were ( 105.8 ± 3.1 )% and ( 99.6 ± 3.6 )%( n =15, RSD : 2.40% ～ 4.87% ) respectively.CONCLUSIONS:This method is able to satisfy demands for measuring glucocorticoid of human urine,and it is very sensitive and credible.The concentration of cortisol and cortisone in 34 healthy volunteers's urine were ( 25.2 ± 14.3 )ng·ml -1 and 46.2 ± 26.7 ng·ml -1 and the ratio of cortisol and cortisone was 0.59 ± 0.18 .

Key concepts: Cortisone, Urine, Glucocorticoid, Endocrinology, Internal medicine, Chemistry, Chromatography, Hydrocortisone

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