Determination of eight synthetic colours in beverage by HPLC-transform wavelength

Objective:To establish a beverage of 8 synthetic colours(tartrazine,sunset yellow,ponceau 4R,amaranth,allura red,erythrosine,indigotine,brilliant blue) wavelengths in the visible light transformation by high performance liquid chromatography Methods.Methods: The sample pH value adjusted to remove carbon dioxide after the ultrasound,and then into the high performance liquid chromatography(HPLC) analysis of the pigment wavelength maximum absorption wavelength in the visible region.Results: The eight kinds of synthetic pigments in the 1.0 mg/L～50mg/L range of good linearity,the correlation coefficient(r) was higher than 0.999,and method recovery was 93.4%～101.5%,the relative standard deviation RSD(%) 1.2%～3.8%.Conclusion: This method is simple,easy to operate,the results were also good,accurate and reliable.