The Effect of Early Enteral Nutrition on the Intestinal Barrier Function of Rats with Severe Acute Pancreatitis

Objective To investigate the influence of early enteral nutrition on the intestinal barrier function of rats with severe acute pancreatitis.MethodsFourty-eight Sprague-Dawley(SD) rats of acute severe pancreatitis were divided into four groups:SAP control(Con)group,traditional parenteral nutrition(TPN) group,enteral nutrition in 24 hours after making model(EN-2) group.enteral nutrition in 12 hours after making model(EN-1) group.SAP was induced in rats by injection of 3.8% deoxycholate into pancrea.sacrifice on the thirdth and sixth day.The vein blood was tested for DAO,TNF-a,and IL-10.Specimens of jejunum were obtained to measure the height of villi and the thickness of mucosa.ResultsCompared with Con group,the levels of DAO,TNF-a,and IL-10 were significantly reduced in EN group at 3 and 6 days after the operation(P0.05).There was decreasing tendency in TPN group than those in con group,but there was not significantly difference(P0.05).There was obviously reduced of the levels of TNF-a,IL-10 and DAO in EN group than those in TPN group(P0.05).Compared with TPN group,the levels of DAO were significantly reduced in EN group(P 0.05).Compared with EN-2 group,the levels of DAO were significantly reduced in EN-1 group(P0.05).The height of the villi and the thickness of mucosa in EN group was higher than those of TPN,Con group at 3 and 6 days after the operation(P0.05).Compared with EN-2 group,the height of the villi and the thickness of mucosa were significantly higher in EN-1 group(P0.05).ConclusionThe results suggested that early enteral nutrition be effective in SAP in improving the gut barrier function and lessen inflammatory reaction.