Inhibitory effect of Curcumin on the expression of cyclin D1 in gastric adenocarcinoma cells

[Objective] To explore the inhibitory effect of curcumin on the expression of cyclin D1 in gastric adenocarcinoma cells.[Methods]⑴Human gastric adenocarcinoma SGC7901 cells were cultured in routine way.Then the expression of transforming growth factor alpha(TGF-α) and epidermal growth factor receptor(EGFR) of cells was detected with immuno-fluorescent staining and flow cytometry(FCM).⑵The SGC7901 cells were cultured with RPMI-1640 without calf serum for 48 h,and then with RPMI-1640 containing 2.5% calf serum.Then cells were treated by TGF-α or curcumin,and simultaneously by TGF-α and curcumin.The cyclin D1 expression of cells was detected by immuno-fluorescent staining and FCM.[Results] ⑴The positive rates of TGF-α and EGFR expression of SGC7901 cells were 46.80% and 57.78 % respectively when cells were cultured in routine way for 48 h.⑵The positive rates of cyclin D1 expression of SGC7901 cells increased by 8.43%(P0.01)when cells were treated by 30 μg/L TGF-α for 5 h,decreased by 21.37%(P0.01)when cells were treated by 20 μmol/L curcumin for 5 h,and decreased by 25.32%(P0.01)when cells were treated simultaneously by TGF-α and curcumin for 5 h.[Conclusion ]The gastric adenocarcinoma SGC7901 cells express TGF-α and EGFR,which suggest that the cells have TGF-α autocrine and paracirne loops for promoting cell proliferation.Curcumin can inhibit the cyclin D1 expression of SGC7901 cells with or without being stimμlated by TGF-α.Curcumin also can inhibit the TGF-α autocrine and paracrine loops for promoting gastric adenocarcinoma cell proliferation.This is one of the ways of curcumin inhibiting cancer cell proliferation.