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2010•Xi'an Jiaotong Daxue xuebaoRequires access

Study of RHD genotypes by the multiplex PCR

He Chen

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Abstract

Objective To establish a reliable PCR method for RHD genotypes research.Methods Based on the characteristics of RHD gene,the specific primers were designed for different RHD genotypes to establish the multiplex PCR and compare it with commercially supplied RHD genotyping kit.Results The multiplex PCR utilized for RHD genotypes was established.The results of RHD genotypes were the same between the multiplex PCR method and commercially supplied kit.Conclusion The method of the multiplex PCR can be utilized for the study of RHD genotypes because it is convenient,accurate and economical.

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What this paper is about

Objective To establish a reliable PCR method for RHD genotypes research.Methods Based on the characteristics of RHD gene,the specific primers were designed for different RHD genotypes to establish the multiplex PCR and compare it with commercially supplied RHD genotyping kit.Results The multiplex PCR utilized for RHD genotypes was established.The results of RHD genotypes were the same between the multiplex PCR method and commercially supplied kit.Conclusion The method of the multiplex PCR can be utilized for the study of RHD genotypes because it is convenient,accurate and economical.

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Available abstract

Objective To establish a reliable PCR method for RHD genotypes research.Methods Based on the characteristics of RHD gene,the specific primers were designed for different RHD genotypes to establish the multiplex PCR and compare it with commercially supplied RHD genotyping kit.Results The multiplex PCR utilized for RHD genotypes was established.The results of RHD genotypes were the same between the multiplex PCR method and commercially supplied kit.Conclusion The method of the multiplex PCR can be utilized for the study of RHD genotypes because it is convenient,accurate and economical.

Key concepts: Multiplex polymerase chain reaction, Genotyping, Multiplex, Genotype, Biology, Genetics, Polymerase chain reaction, Gene

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