Comparing the distrbution of genetic polymorphism of MTHFR reductase between Han populations of Libo and Miao of Leishan in Guizhou.

Objective: The purpose of this study is to compare the polymorphisms of two monoucleotides of methylenetetrahydrofolate Reductase (MTHFR) between the Han populations of Libo and Miao of Leishan in Guizhou to obtain genetic data. Methods: PCR-RFLP technique was used to detect C677T and A 1298C polymorphisms of MTHFR. Results: In Han population, MTHFR C677T the allele frequencies of 677T were 22.7%, which are accordant with the frequency of the Han population of south but lower than nouth of China. They are significantly different between the Han populations and Miao (10.64%) .In addition, For Miao 1298C homozygotes frequencies (48.66%) are higher than those reported by others. The ferquencies of combined heterozygosity for the C677T and A 12989C mutations in Han, Miao were 16.66%, 11.1%. One case of combined homozygote of 677T and 1298C was found in Miao .Conclusion: The two genetic polymorphisms of MTHFR in this study are different between Han populations and Miao of Guizhou; For Miao ,1298C homozygotes frequencies are highest in reported by others.