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Determination of paralytic shellfish poison by means of ELISA and mouse bioassay

Yi‐Wen Huang

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Abstract

Paralytic shellfish poison(PSP) is one of marine biotoxins with world-wide distribution and most serious harmness.In this study,20 samples of Mytilus from Fujian were used to determine PSP by ELISA and mouse bioassay.The results of two methods were consistent,but ELISA was more suitable to detect the Mytilus samples with small PSP content.Comparing these two methods,the mouse bioassay was simple,but couldn't evaluate quantitatively,however,the ELISA assay showed to be simple,sensitive and low cost,which possesses great significance to rapid check out PSP.Combining these two methods could obtain more accurate analyzing results of PSP content.

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What this paper is about

Paralytic shellfish poison(PSP) is one of marine biotoxins with world-wide distribution and most serious harmness.In this study,20 samples of Mytilus from Fujian were used to determine PSP by ELISA and mouse bioassay.The results of two methods were consistent,but ELISA was more suitable to detect the Mytilus samples with small PSP content.Comparing these two methods,the mouse bioassay was simple,but couldn't evaluate quantitatively,however,the ELISA assay showed to be simple,sensitive and low cost,which possesses great significance to rapid check out PSP.Combining these two methods could obtain more accurate analyzing results of PSP content.

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Available abstract

Paralytic shellfish poison(PSP) is one of marine biotoxins with world-wide distribution and most serious harmness.In this study,20 samples of Mytilus from Fujian were used to determine PSP by ELISA and mouse bioassay.The results of two methods were consistent,but ELISA was more suitable to detect the Mytilus samples with small PSP content.Comparing these two methods,the mouse bioassay was simple,but couldn't evaluate quantitatively,however,the ELISA assay showed to be simple,sensitive and low cost,which possesses great significance to rapid check out PSP.Combining these two methods could obtain more accurate analyzing results of PSP content.

Key concepts: Bioassay, Mytilus, Shellfish, Paralytic shellfish poisoning, Biology, Chromatography, Shellfish poisoning, Toxin

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