Effects of Electroacupuncture and Moxibustion on S100β Protein of Acute Cerebral Ischemia-reperfusion Rats

Objective:To investigate the protection BBB by using electro-acupuncture and moxibustion after acute cerebral ischemia reperfusion in rats and the dynamic changes of S100 βprotein level in serum.Method:Use middle cerebral artery occlusion/reperfusion(MCAO/R)rat model.30 minutes later the Baihui and Shuigou points were stimulated by electro-acupuncture and Zusanli point was stimulated by moxbustion.To investigate and evaluate the S100 βprotein level in blood serum after 6h,12 h,24 h,48h of the treatment.Then observe the ultramicrostmcture change of the BBB of each phase before and after the treatment.Results:(1)H-600 transmission electron microscope shows that damage of the BBB system in electro-acupuncture and moxibustion phase are lighter than that in model phase.(2) ELISA results show that S100β protein level in electro-acupuncture and moxibustion group is lower than that in model group(P0.05).Conclusions:Electro-acupuncture and moxibustion can protect the BBB system and cut down the S100 βprotein level in blood serum,keeping the normal physiologic function.