ScholarLens Back to search
2013•Food ScienceRequires access

Preparation and Monosaccharide Composition Analysis of Homogeneous Polysaccharide FVP60-C2 from Fruit Bodies of Flammulina velutipes

Peilong Sun

Open publisher page 0 citations

Abstract

Hot water extraction and ethanol precipitation were used to extract polysaccharide from fruit bodies of Flammulina velutipes.Crude polysaccharide extract was purified to obtain FVP60-C2 by ion exchange chromatography and gel filtration chromatography.The physicochemical properties of FVP60-C2 were investigated by HPLC,UV and IR.Following the hydrolysis and acetylation of FVP60-C2,the monosaccharide compositions of FVP60-C2 were analyzed by GC.The results showed that FVP60-C2 was a homogeneous polysaccharide with molecular weight of 1.429 × 104D,which was composed of fucose,xylose,mannose,glucose and galactose with a molar ratio of 0.74:0.21:1.36:1.00:1.31.

About this research paper

What this paper is about

Hot water extraction and ethanol precipitation were used to extract polysaccharide from fruit bodies of Flammulina velutipes.Crude polysaccharide extract was purified to obtain FVP60-C2 by ion exchange chromatography and gel filtration chromatography.The physicochemical properties of FVP60-C2 were investigated by HPLC,UV and IR.Following the hydrolysis and acetylation of FVP60-C2,the monosaccharide compositions of FVP60-C2 were analyzed by GC.The results showed that FVP60-C2 was a homogeneous polysaccharide with molecular weight of 1.429 × 104D,which was composed of fucose,xylose,mannose,glucose and galactose with a molar ratio of 0.74:0.21:1.36:1.00:1.31.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Hot water extraction and ethanol precipitation were used to extract polysaccharide from fruit bodies of Flammulina velutipes.Crude polysaccharide extract was purified to obtain FVP60-C2 by ion exchange chromatography and gel filtration chromatography.The physicochemical properties of FVP60-C2 were investigated by HPLC,UV and IR.Following the hydrolysis and acetylation of FVP60-C2,the monosaccharide compositions of FVP60-C2 were analyzed by GC.The results showed that FVP60-C2 was a homogeneous polysaccharide with molecular weight of 1.429 × 104D,which was composed of fucose,xylose,mannose,glucose and galactose with a molar ratio of 0.74:0.21:1.36:1.00:1.31.

Key concepts: Flammulina, Polysaccharide, Chemistry, Monosaccharide, Xylose, Chromatography, Ethanol precipitation, Size-exclusion chromatography

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Preparation and Monosaccharide Composition Analysis of Homogeneous Polysaccharide FVP60-C2 from Fruit Bodies of Flammulina velutipes — Research Paper | ScholarLens