Protection of Scalp-acupuncture on Rats with Acute Cerebral Ischemia-reperfusion Injury

Objective:To explore the protection and mechanism of scalp-acupuncture(SA) on acute cerebral ischemia.Methods:Ninety SD rats were divided randomly into group sham-operated,model and SA,and MCAO models were established.The sham-operated group was not resulted in ischemia,and the SA gruop was given SA therapy.At 6,24,48 and 72 h after reperfusion,neurological sevity score(NSS) was used to evaluate the neuralfunction of rats,and to detect apoptosis rate of neuron by TUNEL.To determine the expression of Caspase-3,Bcl-2 and Bax by RT-PCR and Western blot.Results:The NSS score of SA group decreased at 72 h compared with model group(P0.01).The neuron apoptosis rate of model group increased with a few extension of time and reached a peak at 24 h.The neuron apoptosis rate of SA group was less than model group,especially at 24 h and 48 h(P0.01).The expression of Bcl-2 and Bax in ischemia side of brain could reach a peak at 24 h,The mRNA of Bcl-2 and Caspase-3 were significantly different with the model group.Conclusion:Cerebral ischemia can induce high expresssion of Bcl-2,Bax,Caspase-3 gene.SA can protect ischemic brain tissue by inhibiting neuron apoptosis.