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Quantitative analysis of mRNA with RT-PCR

Dai Jian

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Abstract

mRNA of encoding protein was reverse transcripted to be cDNA by polymerase chain reaction (PCR). With the enhancement of PCR,DNA production increased to exponential multiple. And the PCR production was related to the CDNA template or the concentration of mRNA during optimum cycles. After electrophoresis, stained ethidium bromide (EB) into PCR products,the quantity of PCR product was determined by scanning of fluorescent intensity. The relative concentration of mRNA can be calculated by optimizing the experimental condition.

About this research paper

What this paper is about

mRNA of encoding protein was reverse transcripted to be cDNA by polymerase chain reaction (PCR). With the enhancement of PCR,DNA production increased to exponential multiple. And the PCR production was related to the CDNA template or the concentration of mRNA during optimum cycles. After electrophoresis, stained ethidium bromide (EB) into PCR products,the quantity of PCR product was determined by scanning of fluorescent intensity. The relative concentration of mRNA can be calculated by optimizing the experimental condition.

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Available abstract

mRNA of encoding protein was reverse transcripted to be cDNA by polymerase chain reaction (PCR). With the enhancement of PCR,DNA production increased to exponential multiple. And the PCR production was related to the CDNA template or the concentration of mRNA during optimum cycles. After electrophoresis, stained ethidium bromide (EB) into PCR products,the quantity of PCR product was determined by scanning of fluorescent intensity. The relative concentration of mRNA can be calculated by optimizing the experimental condition.

Key concepts: Ethidium bromide, Complementary DNA, Molecular biology, Messenger RNA, Real-time polymerase chain reaction, Polymerase chain reaction, Biology, Primer dimer

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