Determination of phenol in phenol ear drops by ultraviolet spectrophotometry

[Objective]To establish a method for the determination of phenol content in phenol ear drops.[Methods]A series of phenol standard concentration solution was set up,glycerol for blank,the UV spectrophotography was used to determine absorbability for preparing standard curve,the detection wavelength was 270 nm.Absorbability of phenol ear drops and account content was determined by standard curve.[Results] The phenol concentration within 9.6～48.0 μg/ml showed good linear correlation.Other solvent was non-interference.[Conclusions] The method was sample,easy to operate,and available to assay the preparation.