Establishment of the rapid propagation system from stem and leaf explant of Chrysanthemum×grandiflora ‘niu9717'

In vitro micropropagation and shoot regeneration were studied using axillary bud and leaves of Chrysanthemum×grandiflora‘niu9717'aseptic stems as explants.The optimal proliferation medium was MS MS+6-BA 0.3mg·L-1+KT 0.5mg·L-1+NAA 0.5mg·L-1 which had average multiplication coefficient of 3.2.The optimal medium for adventitious bud induction from leaves was MS+6-BA 2.0 mg·L-1+NAA 0.5mg·L-1 which had the callus induction ratio of 100%、adventitious bud differentiation ratio of 92.3%and the reproduction coefficient of 8.9.The combination of 6-BA and NAA was better than that of 6-BA and 2,4-D or others.Cytokinin KT and TDZ were not conducive to callus induction and adventitious bud differentiation.Auxin 2,4-D were conducive to callus induction but not to adventitious bud differentiation.The medium of 1/2MS+IBA 0.05mg·L-1 was the optimal for rooting,which had rooting rate of 100%and the average root number of 12.08and average root length of 1.5cm.The plants after transplanting grew very well with a survival rate of 100%.The high concentration of auxin could induce root but inhibit elongation.