The immune responses induced by the DNA vaccine expressing fusion protein of HSV-2gD-HBsAg and IL-18

Objective:To evaluate humoral and cellular immune responses induced by the DNA vaccine expressing fusion protein of HSV-2gD-HBsAg and IL-18 in BALB/c mice.The P6 is mimotope of HSV-2gD,the NP6 is the sequence of natural HSV-2gD which is similar to P6.Methods:The plasmid pcDNA3.1(negative control) and the recombinant plasmids pc-S-P6-IL18 and pc-S-NP6-IL18 were inoculated into BALB/c mice by intramuscular injection for three times,twice a week.Two weeks after the last vaccination,the level of specific IgG antibody,IFN-γ and IL-18 were measured by ELISA;one month after the last vaccination,spleen cells of vaccinated mice were separated and the proliferative response of spleen lymphocytes was stimulated by ConA and measured by MTT.Results:After inoculated by pc-S-P6-IL18 and pc-S-NP6-IL18 vaccines,the mice could produce higher level of splenocyte proliferative response and secrected IFN-γ,IL-18 and specific antibody than by pcDNA 3.1.Conclusion:The recombinants of pc-S-P6-IL18 and pc-S-NP6-IL18 can effectively induce humoral and cellular immune responses,which can offered the basic study for preventing against the infections of hepatitis B virus and herpes simplex virus type 2.