Expression of HIF-1α Gene Mediated by Adenovirus in Ischemic Myocardium

Hypoxia-inducible factor 1(HIF-1) is a transcription factor that is composed of α and β subunits. HIF-1 activity depends on the amout of HIF-1α subunit, which is tightly regulated by the oxygen tension. To study the expression about HIF-1α mRNA in ischemic myocardium after acute myocardiac infarction(AMI), the animal AMI model were made by rabbits, divided into 4 groups at random and infected Ad-HIF-1α,Ad-Blank,Rz-HIF-1α respectively,Sham group was regarded as control. The mRNA and protein expression of HIF-1α and VEGF was detected with RT-PCR and immunohistochemistry at different time point. The mRNA and protein expression of HIF-1α and VEGF was increased at 1 day after AMI,reached the peak at 7 days and decreased gradually at 14 days、28 days. At 56 days, HIF-1α mRNA and protein was not expressed, but VEGF mRNA and protein was still expressed. These results support that Ad-HIF-1α is transducted effectively and activated in ischemic myocardium. The time window of Ad-HIF-1α expression is from acute stage to subacute stage after AMI. The expression of Rz-HIF-1α can suppressed in expression of HIF-1α and its downstream gene.