Apoptosis of BcaCD885 Cell Mediated by Human bax Gene

Objective To study the change of apoptosis by bax gene in human buccal mucosa carcinoma BcaCD885 cells. Methods Transfected recombinant human Bax gene plasmid and contral plasmid into BcaCD885 cells. The BcaCD885 cells were examined morphologically through haematoxylin-eosinophil (HE) staining. The apoptosis qualification of the BcaCD885 cells was examined through terminal deoxynucleotidyl transferase-mediated dUTP nick-end labelling technique (TUNEL). The apoptosis rate of the BcaCD885 cells was measured with flow cytometry. Results The bax gene transfected BcaCD885 cells had apoptosis morphological features. Many TUNEL positive red staining cells were found in the bax gene transfected BcaCD885 cells. There were significant differences in the apoptotic rates between the bax gene transfected BcaCD885 and the control BcaCD885 cells and the control plasmid transfected BcaCD885 cells (P 0.01 ). Conclusion The recombinant human bax gene plasmid pORF-h bax-α can promote the apoptotic rate of the BcaCD885 cells.