Role of bone marrow mesenchymal stem cells in severe acute pancreatitis in rat

Purpose To investigate the role of bone marrow mesenchymal cells in severe acute pancreatitis(SAP) in rat.Methods SAP model was induced by receiving intraperitoneal injection with caerulein and lipopolysaccharide(LPS).Rat MSCS were isolated and cultured in vitro,stained with Hoechst33258 and transplanted in rats.The level of plasma TNF-a and IL-6 was detected at 0.5,2,4,6,12 h after caerulein and LPS injection.The pathological changes of pancreas and lung,and the distribution of stained MSCS in pancreas lung and liver were detected at the same time.Results In comparison with control group,the plasma cytokine of TNF-α,IL-6 expression was suppressed,and lung wet dry ratio lowered at different time points after injection of MSCS.The injury of pancreas and lung was reduced.The difference of distribution of stained MSCS between these organs is significant.Conclusion MSCS have significantly protective effects in SAP of rat.